Some aspects of data analysis of multi-angle dynamic light scattering

2000 ◽  
Vol 162 (1) ◽  
pp. 173-190 ◽  
Author(s):  
Ralf Buttgereit ◽  
Michael Marth ◽  
Tobias Roths ◽  
Josef Honerkamp
Keyword(s):  
Data Analysis ◽  
Light Scattering ◽  
Dynamic Light Scattering

scholarly journals Towards Reproducible Enzyme Modeling with Isothermal Titration Calorimetry

2021 ◽  
Author(s):  
Felix Ott ◽  
Kersten S. Rabe ◽  
Christof M Niemeyer ◽  
Gudrun Gygli
Keyword(s):  
Quality Control ◽  
Data Analysis ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Isothermal Titration Calorimetry ◽  
Molecular Mechanisms ◽  
Kinetic Studies ◽  
Titration Calorimetry ◽  
Kinetic And Thermodynamic Parameters ◽  
Enzyme Modeling

<div> <p>An experimental workflow to provide detailed information of the molecular mechanisms of enzymes is described. This workflow will help in the application of enzymes in technical processes by providing crucial parameters needed to plan, model and implement biocatalytic processes more efficiently. These parameters are homogeneity of the enzyme sample (HES), kinetic and thermodynamic parameters of enzyme kinetics and binding of reactants to enzymes. The techniques used to measure these properties are dynamic light scattering (DLS), UV-Vis spectrophotometry and isothermal titration calorimetry (ITC) respectively. The workflow is standardized by the use of SOPs and python-scripted data analysis. </p> <p>We have used the NADPH-dependent alcohol dehydrogenase Gre2p as a challenging enzyme to demonstrate the power of this workflow. Our work highlights the utility for combined binding and kinetic studies for such complex multi-substrate reactions and the importance of sample quality control during experiments.</p> </div>

scholarly journals Towards Reproducible Enzyme Modeling with Isothermal Titration Calorimetry

2021 ◽  
Author(s):  
Felix Ott ◽  
Kersten S. Rabe ◽  
Christof M Niemeyer ◽  
Gudrun Gygli
Keyword(s):  
Quality Control ◽  
Data Analysis ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Isothermal Titration Calorimetry ◽  
Molecular Mechanisms ◽  
Kinetic Studies ◽  
Titration Calorimetry ◽  
Kinetic And Thermodynamic Parameters ◽  
Enzyme Modeling

<div> <p>An experimental workflow to provide detailed information of the molecular mechanisms of enzymes is described. This workflow will help in the application of enzymes in technical processes by providing crucial parameters needed to plan, model and implement biocatalytic processes more efficiently. These parameters are homogeneity of the enzyme sample (HES), kinetic and thermodynamic parameters of enzyme kinetics and binding of reactants to enzymes. The techniques used to measure these properties are dynamic light scattering (DLS), UV-Vis spectrophotometry and isothermal titration calorimetry (ITC) respectively. The workflow is standardized by the use of SOPs and python-scripted data analysis. </p> <p>We have used the NADPH-dependent alcohol dehydrogenase Gre2p as a challenging enzyme to demonstrate the power of this workflow. Our work highlights the utility for combined binding and kinetic studies for such complex multi-substrate reactions and the importance of sample quality control during experiments.</p> </div>

Dynamic light scattering from gels in a poor solvent

1978 ◽  
Vol 39 (9) ◽  
pp. 955-960 ◽  
Author(s):  
E. Geissler ◽  
A.M. Hecht
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Poor Solvent

DYNAMIC LIGHT SCATTERING IN LC-440 NEMATIC LIQUID CRYSTAL

2014 ◽  
Vol 73 (11) ◽  
pp. 977-983
Author(s):  
A. P. Fedoryako ◽  
A. I. Kocherzhin ◽  
M. P. Kukhtin ◽  
E. I. Chernyakov
Keyword(s):  
Liquid Crystal ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Nematic Liquid Crystal

Synthesis of Calcium Carbonate Micro Particles using Emulsion Membrane Process Applied for Drug Release Studies

2017 ◽  
Vol 68 (10) ◽  
pp. 2320-2324
Author(s):  
Mariana Mateescu ◽  
Sanda Maria Doncea ◽  
Irina Chican ◽  
Cristina Lavinia Nistor ◽  
Ioneta Codrina Bujanca
Keyword(s):  
Light Scattering ◽  
Calcium Carbonate ◽  
Dynamic Light Scattering ◽  
Liquid Membranes ◽  
Membrane Process ◽  
Emulsion Method ◽  
Micro Particles ◽  
Release Studies ◽  
Emulsion Liquid Membranes ◽  
Span 80

The aim of this work is the synthesis of calcium carbonate (CaCO3) nano and microparticles and their application as biomaterials (vehicles) for the sustained release of doxycycline. CaCO3 micro particles were synthesized by water-in oil (W/O) emulsion method using emulsion liquid membranes with bis (2-ethylhexyl) phosphate (D2EHPA) as carrier, Span 80 as surfactant, and toluene and kerosene as organic solvents. The aqueous phases contained 1 M CaCl2, and 1 M Na2CO3, respectively. The Dynamic Light Scattering (DLS) data showed CaCO3 particles with sizes ranging from around 100 nm to 3500 nm. The CaCO3 particles with the average diameters around 600 nm attained an adsorbtion of doxycycline of maximum 97.9%, and a slow and steady release with a cumulative value of approximative 50% after ten days.

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